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KMID : 0379520040200040307
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한국독성학회지
2004 Volume.20 No. 4 p.307 ~ p.313
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MISTLETOE (Viscum album var. coloratum) Growing on Carpinus laxiflora BL. Induces the Differentiation of Human Acute Promyeocytic Leukemia (HL-60) Cells
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Kim Sang-Cheol
Park Soo-Young
Hyoun Jae-Hee
Cho Hee-Yeong
Lee Young-Jae
Kang Ji-Hoon
Lee Young-Ki
Park Deok-Bae
Yoo Eun-Sook
Kang Hee-Kyoung
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Abstract
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The present study was undertaken to investigate the effects of mistletoe (Viscum album var. coloratum) growing on Carpinus laxiflora BL. on proliferation and differentiation of HL-60 acute promyelocytic leukemia cells. Aqueous extract and its (NH_2)_2SO_4 saturated fractions of the mistletoe exhibited potent anti-proliferation activity against HL-60 cells. Moreover, when HL-60 cells were treated with 0~30% and 30～70% (NH_2)_2SO_4 saturated fractions of the mistletoe, HL-60 expressed CD 66b or CD 14 cell surface antigens and showed activity to reduce nitroblue tetrazolium, indicating that mistletoe induces the differentiation of HL-60 into granulocytes or monocytes. To understand how mistletoe induces the differentiation, we investigated the expression of molecules for modulating the proliferation and differentiation of leukemia cells, such as c-Myc and myeloblastin. The 0~30% (NH_2)_2SO_4 saturated fraction of the mistletoe reduced the mRNA levels of c-Myc and myeloblastin in a time-dependent manner. The results indicate that the mistletoe induces the differentiation of HL-60 cells via the decrease of c-Myc and myeloblastin expressions. Thus, it is suggested that mistletoe has a therapeutic potential for the treatment of acute promyelocytic leukemia.
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KEYWORD
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HL-60 cells, Differentiation, Mistletoe, c-Myc, Myeloblastin
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